Microorganism detection and identification using amplified single molecule detection

Using an amplified single molecule detection platform based on padlock probe and proximity ligation, detection of very low amounts of nucleic acid or protein molecules is achieved. Protein and nucleic acid analyses are performed in a similar fashion using circularizing DNA probes, rolling circle amplification and fluorescence-based counting of single reporter molecules. This allows a single system to be used for detection and identification of different types of biological threats, such as bacteria, bacterial spores, viruses, and toxins.